Original Research

Expression of DC-SIGN and DC-SIGNRs in placentas of HIV-positive patients

Komala Pillay, M Cloete, H McLeod
Southern African Journal of HIV Medicine | Vol 15, No 3 | a8 | DOI: https://doi.org/10.4102/sajhivmed.v15i3.8 | © 2014 Komala Pillay, M Cloete, H McLeod | This work is licensed under CC Attribution 4.0
Submitted: 12 December 2014 | Published: 08 September 2014

About the author(s)

Komala Pillay, Department of Anatomical Pathology, University of Cape Town, and Red Cross Children’s War Memorial Hospital, Cape Town, South Africa, South Africa
M Cloete, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, University of Cape Town, South Africa, South Africa
H McLeod, Department of Anatomical Pathology, University of Cape Town, and Red Cross Children’s War Memorial Hospital, Cape Town, South Africa, South Africa

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Abstract

Background. Human dendritic cell-specific intracellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) is a mannose-binding lectin that initiates interaction between dendritic cells and resting T-lymphocytes. DC-SIGN is highly expressed in placental tissue on dendritic cells and Hofbauer cells, and it is suggested that HIV may become adsorbed to DC-SIGN on Hofbauer cells as part of the mechanism of mother-to-child HIV transmission. A possible mechanism of transfer of the virus from the Hofbauer cells to the fetus is the subsequent adsorption to DC-SIGN-related molecules (DC-SIGNRs), present on immediately adjacent capillary vascular endothelium. However, data on DC-SIGN and DC-SIGNR expression in the placenta are few.

Methods. Forty term placentas from HIV-positive mothers and 21 term placentas from HIV-negative mothers underwent immunohistochemistry staining for DC-SIGN and DC-SIGNR expression. Five random sets of 10 villi were assessed, and the average number of positive cells were counted in each case. In addition, where possible, maternal and cord blood viral loads and maternal CD4+ counts were performed in the HIV-positive group only.

Results. The median maternal CD4+ count was 377 cells/µl and 27% of participants had undetectable viral loads; the median detectable viral load was 3.72 log. Most (97%) of the cord bloods tested in infants from HIV-positive mothers had lower than detectable viral loads. HIV-positive cases had significantly greater expression of both DC-SIGNRs (median values in HIV-positive cases, 14.5 positive cells/10 villi (pc/10villi), compared with 11 pc/10villi in HIV-negative cases, p=0.020) and DC-SIGN (median value in HIV-positive cases, 26.5 pc/10villi, compared with 23 pc/10villi in HIV-negative cases, p=0.037). DC-SIGNR expression was also noted in Hofbauer cells and decidual macrophages in addition to endothelium (reported currently). There was no difference in expression of DC-SIGN and DC-SIGNRs in patients with or without chorioamnionitis, but there was an inverse relationship between DC-SIGN and DC-SIGNR expression and maternal CD4+ counts in HIV-positive cases. 

Conclusion. Both DC-SIGN and DC-SIGNR expression were higher in placentas from HIV-positive mothers compared with HIV-negative cases. These lectins may be potential new therapeutic targets for preventing vertical transmission of HIV.


Keywords

HIV; placenta; virus transmission; DC-SIGN; DC-SIGNR

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